Амплификация области гена МВ, содержащей тандемные повторяющиеся последовательности.

Эти праймеры были использованы для ПЦР во всех сочетаниях, однако нам не удалось получить ампликоны теоретической расчетной длины во всех случаях. После серии опытов была выбрана пара праймеров MBS387 и UMA1213 как наиболее подходящая для получения продуктов амплификации области МВ гена, содержащей тандемные повторяющиеся последовательности. В общей сложности из 18 образцов ДНК, выделенных из разных клинических изолятов, получить ампликоны удалось только в 6 случаях (рис 12):

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